Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Aptamer Detection of Mycobaterium tuberculosis Mannose-Capped Lipoarabinomannan in Lesion Tissues for Tuberculosis Diagnosis

doi: 10.3389/fcimb.2021.634915

Figure Lengend Snippet: Representative images and comparison between acid-fast staining and ManLAM-based immunohistochemistry (IHC) on lesion tissues. Representative images of (A) lesion sections of intestinal tuberculosis (TB) patient, (B, C) tissues of lymph node TB patients, and (D) lesion sections of body lumbar TB. TB, tuberculosis. Black arrows indicate positive signals (AFS by red staining, or aptamer-based IHC by brown granular staining). Samples with a score less than the cutoff value (0.0580) were designated as “-”, (≥0.0580 and <0.5010) as “+”, (≥0.5010 and <0.8780) as “++”, >0.8780 as “+++”. The numbers (0.5010 and 0.8780) were determined by calculating the 50% and 75% percentile of the Scores.

Article Snippet: The AFS kits using Ziehl-Neelsen (ZN) staining were purchased from Baso Diagnostics Inc (Zhuhai, China).

Techniques: Comparison, Staining, Immunohistochemistry

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Aptamer Detection of Mycobaterium tuberculosis Mannose-Capped Lipoarabinomannan in Lesion Tissues for Tuberculosis Diagnosis

doi: 10.3389/fcimb.2021.634915

Figure Lengend Snippet: Representative images and comparison between acid-fast staining (AFS) and ManLAM aptamer-based IHC in lesion tissues with negative AFS. Representative images of (A) tissues of pulmonary tuberculosis (TB) patient, (B) lesion sections of lymph node TB patient, (C) lesion sections of LTBI patient, (D) lesion sections of non-TB patient, (E, F) lesion sections of two NTM patients. TB, tuberculosis. NTM, nontuberculous mycobacteria. Arrows indicate positive signals (aptamer-based immunohistochemistry (IHC) by brown granular staining; AFS by red staining). Samples with a score less than the cutoff value (0.0580) were designated as “-”, (≥0.0580 and <0.5010) as “+”, (≥0.5010 and <0.8780) as “++”, >0.8780 as “+++”. The numbers (0.5010 and 0.8780) were determined by calculating the 50% and 75% percentile of the Score.

Article Snippet: The AFS kits using Ziehl-Neelsen (ZN) staining were purchased from Baso Diagnostics Inc (Zhuhai, China).

Techniques: Comparison, Staining, Immunohistochemistry

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Aptamer Detection of Mycobaterium tuberculosis Mannose-Capped Lipoarabinomannan in Lesion Tissues for Tuberculosis Diagnosis

doi: 10.3389/fcimb.2021.634915

Figure Lengend Snippet: Comparison of aptamer- and antibodies-based immunohistochemistry (IHC)/immunofluorescence for tuberculosis (TB) diagnosis in TB mouse model. Images of M.tb H37Rv infected mouse lung sections (A) and uninfected mouse lung sections (B) detected by acid-fast staining (AFS), aptamer-based IHC, anti-ManLAM, anti-Ag85B, or anti-Rv2645 pAbs. Arrows indicate positive signals (aptamer-based IHC by brown granular staining; AFS by red staining). (C) . Images of infected mouse lung sections detected by AF488 labeled ManLAM aptamer-based immunofluorescence, anti-Rv1579, anti-Rv2645 pAbs developed with AF488-anti-IgG and Rhodamine B (RB). RB is a kind of M.tb specific fluorescence dye, used as a positive control. Samples with a score less than the cutoff value (0.0580) were designated as “-”, (≥0.0580 and <0.5010) as “+”, (≥0.5010 and <0.8780) as “++”, >0.8780 as “+++”. The numbers (0.5010 and 0.8780) were determined by calculating the 50% and 75% percentile of the Score.

Article Snippet: The AFS kits using Ziehl-Neelsen (ZN) staining were purchased from Baso Diagnostics Inc (Zhuhai, China).

Techniques: Comparison, Immunohistochemistry, Immunofluorescence, Biomarker Discovery, Infection, Staining, Labeling, Fluorescence, Positive Control